Protein Expression & Purification

Recombinant Protein production is a powerful technique used in the life sciences. The ability to express and purify a desired recombinant protein in large scale will allow scientists to explore a wide range of possibilities in protein research.

Aurora Biolabs services can take your project directly from gene synthesis to protein expression and recombinant protein purification. Protein products usually are purified with three different columns including affinity, ion exchange, and size-exclusion. The purity of the final products is greater than 95% and can be directly used for crystallization and other high quality assays.

Specific activities and conformational homology of a protein are also major issues we consider during purification. We not only use general protecting reagents during the purification process, but also add various protection additives based on published literatures about the protein. Protein characterization of the final product can be tested in house or at the local Bioscience Service Center using Electrophoresis, Bioactivity study, Reversed-phase HPLC, Dynamic Light Scattering, Mass Spectroscopy Instruments, and BioCore.

Combining Fermenter, Akta Explorer, and large sized chromatography systems in the laboratory, we can produce quantities of protein samples ranging from 10 mgs to a few grams.

For more information and quotation, please call (858) 215-4510 or e-mail us at: info@aurorabiolabs.com, or use our contact page: Contact