IDO1 Activity Assay Kit for Inhibitor Screening
Tryptophan is an essential amino acid that is involved in protein synthesis and regulation of the local immune response by T lymphocytes. Indoleamine 2,3-dioxygenase-1 (IDO1) catalyzes oxidation of tryptophan to N-formylkynurenine (NFK), the initial and rate limiting step in the pathway of catabolism of tryptophan. Over expression of IDO1 in variety of cancers results in the depletion of Tryptophan and the accumulation of kynurenine, that have been proposed as mechanisms that contribute to the suppression of the immune response.
IDO1 Activity Assay Kit is designed to measure the activity of IDO1 enzyme and can be used for screening IDO1 inhibitors. The activity assay is carried out on a 96-well plate. After incubation of the enzyme, substrate and inhibitors, absorbance of the product NFK is measure at 321nm, and IDO1 activity is calculated based on the absorbance value. The kit contains enough solutions for 100 reactions.
