TEV Protease Activity Assay Kit
Tobacco Etch Virus protease (TEV protease) is a highly sequence specific cysteine protease. It has a strict 7 amino acid cleavage recognition sequence of Glu-Asn-Leu-Tyr-Phe-Gln ↓ (Gly/Ser). The high specificity makes this protease excellent for the removal of affinity-tags from purified recombinant proteins.
The TEV Protease Activity Assay kit is a fluorogenic-based assay to measure TEV protease activity. The kit contains a TEV protease substrate that is labeled with fluorophore FAM and a quencher. Proteolytic activity of TEV protease cleaves the substrate and releases the FAM, resulting in the production of bright fluorescence which can be measured using a fluorescence reader at ex/em of 490 nM/520 nm. TEV protease activity then can be calculated in accordance with the fluorescence intensity. Purified TEV protease is included in the kit as a positive control.
