Scientific Details
AA Sequence:
FNLPPGNYKKPKLLYCSNGGHFLRILPDGTVDGTRDRSDQHIQLQLSAE SVGEVYIKSTETGQYLAMDTDGLLYGSQTPNEECLFLERLEENHYNTYIS KKHAEKNWFVGLKKNGSCKRGPRTHYGQKA ILFLPLPVSSD
Endotoxin:
< 1.0 EU/ μg of protein as determined by the LAL assay
Bioactivity:
ED50 < 0.1 ng/ml as determined by its ability to the dose dependent proliferation of mouse Balb/c 3T3 cells.
Formulation:
Sterile filtered through a 0.2 micron filter. Lyophilized with no additives.
Purity:
Greater than 98%, as determined by SDS-PAGE
Reconstitution:
Centrifuge the vial before opening. Reconstitute in sterile water to aconcentration of 0.1 -1.0 mg/ml. Do not vortex. For extended storage, it isrecommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.
Source:
Escherichia Coli.
Storage & Stability:
This product is shipped at 4 °C with cold pack. It is stable for up to 6months from date of receipt when stored at -20°C. Multiple freeze/thawcycles should be avoided as it can result in significant loss of activity.
| Catalog # | Product Name |
|---|---|
| 12-0004CR | |
| 12-0004CFR | |
| 12-0008 |
References
1. Expression of acidic fibroblast growth factor (aFGF) and fibroblast growth factor receptor 4 (fgfr4) in breast fibroadenomas. J. Clin. Pathol., Jan 2001, 54: 37 -41.
2. Requirement of phosphatidylinositol 3-kinase activity for translocation of exogenous aFGF to the cytosol and nucleus. J. Biol. Chem., Apr 2000, 275: 11972.
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