Scientific Details
Endotoxin:
Less than 0.01 ng/μg cytokine as determined by the LAL assay
Bioactivity:
Human LIF activity is assessed by its ability to induce differentiation of M1 myeloid leukemia cells. The specific activity is 1 x 106 units/ml and 1 x 107 units/ml, where 50 units is defined as the amount of human LIF required to induce differentiation in 50% of the M1 colonies in 1 ml of medium.
Formulation:
1x phosphate-buffered saline with 0.02% Tween-20. Sterile filtered through a 0.2 micron filter.
Purity:
Greater than 97%, as determined by SDS-PAGE (see Fig. 1)
Source:
Escherichia Coli.
Storage & Stability:
This product is shipped at ambient temperature with cold pack. It is stable for 6 to 12 months from date of receipt when stored at 2-4 °C. For extended storage, it is recommended to store in working aliquots at -20°C to -80°C. Freeze-thaw should be avoided as it can result in loss of activity.
Suggested Usage:
1 ml of human LIF with the specific activity of 1x106 units/ml is sufficient to treat 1 L of embryonic stem cell culture media. 1 ml of human LIF with the specific activity of 1x107 units/ml is sufficient to treat 10 L of embryonic stem cell culture media.
| Catalog # | Product Name |
|---|---|
| 12-0004CFL |
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