
Aurora-Butyl-RF HIC Resin is made with highly cross-linked agarose beads with average particle size of 90 μm optimized for rapid flow (RF).
Principle of HIC (Hydrophobic Interaction Chromatography)
Hydrophobic Interaction Chromatography is a chromatographic method that utilizes the interaction between hydrophobic groups carried by biomolecules and hydrophobic ligands of the stationary phase to separate molecules. During the purification process, the presence of salt ions disrupts the hydration film on the surface of biomolecules and promote binding of biomolecular hydrophobic groups to ligands. Butyl is one of the commonly used ligands.