Enterokinase Activity Assay Kit
The Enterokinase (EK) Activity Assay Kit is a fluorogenic assay designed to quantitatively measure EK protease activity. The kit includes a specific EK substrate (Asp-Asp-Asp-Asp-Lys) conjugated to the fluorophore AFC (7-amino-4-trifluoromethylcoumarin). Upon proteolytic cleavage of the substrate by EK, free AFC is released, generating a strong fluorescent signal. The fluorescence can be detected using a microplate fluorescence reader with excitation at 365 nm and emission at 505 nm. The increase in fluorescence intensity is directly proportional to EK enzymatic activity and can be used to calculate protease activity. Purified EK protease is provided in the kit as a positive control to validate assay performance and ensure proper assay setup.
