Scientific Details
AA Sequence:
AAGSITTLPALPEDGGSGAFPPGHFKDPKRLYCKNGGFFLRIHPDGRVDGVREKSDPHIKLQLQAEERGVVSIKGVCANRYLAMKEDGRLLASKCVTDECFFFERLESNNYNTYRSRKYTSWYVALKRTGQYKLGSKTGPGQKAILFLPMSAKS
Endotoxin:
Less than 0.01 ng/μg cytokine as determined by the LAL assay
Bioactivity:
ED50= 0.1-1.0 ng/ml as determined by the dose dependent proliferation of NIH 3T3 cells (figure-2, 3).
Formulation:
Sterile filtered through a 0.22 micron filter with Tris buffer (10 mM Tris pH7.5, 150 mM NaCl).
Purification:
Sequential chromatography
Purity:
Greater than 98% as determined by SDS-PAGE (figure-1)
Source:
Escherichia Coli
Storage & Stability:
The product is shipped on dry ice. Storage use a manual defrost freezer and avoid repeated freeze-thaw cycles. It is stable for 12 months from date of receipt at -20 °C to -80 °C or two weeks at 2 °C to 8 °C under sterile conditions.
size:
5 µg, 10 µg, 25 µg, 50 µg, 100 µg, 250 µg, 1.0 mg
Suggested Usage:
Useful for cell culture and for the study of signaling pathways.
Catalog # | Product Name |
---|---|
12-0008 | |
12-0011 | His-tagged Human Eukaryotic Translation Initiation Factor (EIF4E) Complexed With EIF4G |
12-0004CFR | |
12-0004CFL | |
12-0012 | Human Eukaryotic Translation Initiation Factor (EIF4E) Complexed With m7GTP |
12-0010 | His-tagged Human Eukaryotic Translation Initiation Factor 4E (EIF4E) |
12-0004CR |
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