Human FGF-basic: Formulation II-Carrier-Lyophilized

Recombinant human Fibroblast Growth Factor-basic (bFGF) (AA134-288), also called as FGF-2 or HBGF-2, is a bioactive protein intended for use in cell culture applications. bFGF is a heparin-binding member of the FGF superfamily of molecules. It is involved in a number of biological processes including embryonic development, differentiation, survival, regeneration and migration (1-5). In addition, bFGF is a critical factor for growing embryonic stem cells in culture to remain in an undifferentiated state. Recombinant human FGF-basic is a 17.3 kDa protein consisting of 154 amino acid residues.

Figure 1.    SDS Page of Recombinant Human bFGF Sample.
Figure 2a.  NIH/3T3 (mouse fibroblast cell line, ATCC: CCL-1668) were cultured before adding bFGF.
Figure 2b.  NIH/3T3 (mouse fibroblast cell line, ATCC: CCL-1668) were cultured with 0.25 ng/ml in serum-free medium at 37 °C, 5% CO2 for 24 hours.
Figure 3.    bFGF-2 Activity Assay. The optical density (OD) of each concentration of the testing samples, which is directly proportional to the cell number, was read at 492 nm at the endpoint of incubation. The cell proliferation dose curve was plotted with OD 492 vs. FGF basic concentrations (ng/mL). Figure 4. iPS cell generation using the three different formulations of bFGF. Figure 5. bFGF samples in tubes.