Mpro of Severe acute respiratory Coronavirus 2 (SARS CoV-2 Mpro, also referred to as SARS-CoV-2 Main protease, SARS-CoV-2 3CL protease) plays an essential role in viral replication by processing the polyproteins 1ab at 11 cleavage sites. Inhibiting the activity of this enzyme would block viral replication, therefore, it becomes a promising target for the development of anti-coronaviral therapeutic agents. Aurora SARS-CoV-2 Mpro assay kit is a homogeneous FRET-based assay for screening the Mpro Inhibitors. The assay is fast and convenient, and it just requires two steps. In the first step, the Mpro enzyme is preincubated with the compound for 30 minutes. The reaction is started by adding substrate solution at the second step. The fluorescent intensity is measured with a fluorescent plate reader at the excitation wavelengths of 340-360 mm and emission wavelengths of 460-480 mm. Materials Supplied: 2x Assay Buffer (20ml), 0.5M DTT (200ul), 0.5 mM Substrate (10ul), Recombinant SARS-CoV-2 Mpro (5ug), and one 96 well plate Materials needed but not supplied: A microplate reader capable of measuring fluorescence at excitation wavelengths of 340-360 mm and emission wavelengths of 460-480 mm. *For a complete list of information, please see the attached documents*

SARS-CoV-2 Mpro Assay Kit Information